NPM1 [p Thr199] Antibody (24GB95)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50502

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB95

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NPM1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-NPM1 (T199) (Uniprot: P06748)

Modification

p Thr199

Specificity

This antibody detects endogenous levels of NPM1 only when phosphorylated at T199

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPM1 [p Thr199] Antibody (24GB95)

NPM1 [p Thr199] Antibody (24GB95)

Immunocytochemistry/ Immunofluorescence: NPM1 [p Thr199] Antibody (24GB95)[NBP3-50502] -

Immunocytochemical staining of HepG2 cells with NPM1 antibody (1:1,000). Nuclei were stained blue with DAPI; NPM1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
NPM1 [p Thr199] Antibody (24GB95)

Flow Cytometry: NPM1 [p Thr199] Antibody (24GB95)[NBP3-50502] -

Flow cytometric analysis of NPM1expression in HepG2 cells using NPM1 antibody (1:2,000). Green, isotype control; red, Phospho-Nucleophosmin (T199).
NPM1 [p Thr199] Antibody (24GB95)

Western Blot: NPM1 [p Thr199] Antibody (24GB95)[NBP3-50502] -

Western blotting analysis using NPM1 (T199) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NPM1 [p Thr199] Antibody (24GB95)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NPM1

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 [p Thr199] Antibody (24GB95)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NPM1 [p Thr199] Antibody (24GB95)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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