Nox4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-58851
![Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Immunohistochemistry-NB110-58851-img0005.jpg "Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]")
![Western Blot: Nox4 AntibodyBSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Western-Blot-NB110-58851-img0015.jpg "Western Blot: Nox4 AntibodyBSA Free [NB110-58851]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Primate
Cited:
Human, Mouse, Rat, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence. [Swiss-Prot# Q9NPH5].
Localization
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nox4 Antibody - BSA Free
Western Blot: Nox4 AntibodyBSA Free [NB110-58851]
Western Blot: Nox4 Antibody [NB110-58851] - Whole cell protein from human HeLa, Hek293, HepG2, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml anti-Nox4 in 1% BSA and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Nox4 is detected at approx. 70 kDa (arrowhead)![Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58851-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]")
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]
Immunocytochemistry/Immunofluorescence: Nox4 Antibody [NB110-58851] - HeLa cells were fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with antibodies to Nox4 (NB110-58851) and the ER marker Calreticulin (NBP1-47518), each at 20 ug/ml for 1 hour at room temperature. The coverslips were washed 3x in PBS and incubated with Alexa-Fluor 488 anti-rabbit secondary antibody. (green) and DyLight 550 anti-mouse secondary antibody. The merged image shows the co-localization of Nox4 and Calreticulin in the ER.![Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Immunohistochemistry-NB110-58851-img0019.jpg "Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]")
Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]
Nox4-Antibody-Immunohistochemistry-NB110-58851-img0019.jpg![Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58851-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]")
![Western Blot: Nox4 AntibodyBSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Western-Blot-NB110-58851-img0020.jpg "Western Blot: Nox4 AntibodyBSA Free [NB110-58851]")
![Western Blot: Nox4 AntibodyBSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Western-Blot-NB110-58851-img0016.jpg "Western Blot: Nox4 AntibodyBSA Free [NB110-58851]")
![Western Blot: Nox4 AntibodyBSA Free [NB110-58851]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Western-Blot-NB110-58851-img0021.jpg "Western Blot: Nox4 AntibodyBSA Free [NB110-58851]")
Applications for Nox4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 - 20 ug/ml
Immunohistochemistry
10 - 20 ug/ml
Immunohistochemistry-Paraffin
10 - 20 ug/ml
Immunoprecipitation
reported in scientific literature (PMID 25062272)
Western Blot
2 - 4 ug/ml
Application Notes
In Western Blot this NOX4 antibody recognizes bands at ~70kDa. See Simple Western Antibody Database for Simple Western validation: Tested in pancreas; separated by size; antibody dilution of 1:1000.
Reviewed Applications
Read 2 reviews rated 3.5 using NB110-58851 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nox4
Long Name
NADPH Oxidase 4
Alternate Names
KOX-1, RENOX
Gene Symbol
NOX4
Additional Nox4 Products
Product Documents for Nox4 Antibody - BSA Free
Product Specific Notices for Nox4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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