Notch-3 Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF1559CL1
![Notch-3 Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af1559cl1_sheep-notch-3-pab-corafluor-1-311220251522570.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Blockade of Receptor-ligand Interaction
Label
CoraFluor 1
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47
Ala40-Glu467
Accession # Q9UM47
Specificity
Detects human Notch-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Notch-3 is observed and less than 5% cross-reactivity with recombinant human (rh) Notch-1 and rhNotch-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Notch-3 Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Notch-3 Antibody [CoraFluor™ 1]
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF1559. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Notch-3
Alternate Names
Notch3
Gene Symbol
NOTCH3
Additional Notch-3 Products
Product Documents for Notch-3 Antibody [CoraFluor™ 1]
Product Specific Notices for Notch-3 Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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