Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Nogo-A Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA. The immunogen is located within the first 50 amino acids of NogoA. Amino Acid Squence: SDSPPRPQPAFKYQFVREPEDEE

Specificity

At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Nogo-A Antibody - BSA Free (NBP2-41070) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nogo-A Antibody - BSA Free

Western Blot: Nogo-A AntibodyBSA Free [NBP2-41070]

Western Blot: Nogo-A AntibodyBSA Free [NBP2-41070]

Western Blot: Nogo-A Antibody [NBP2-41070] - Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunohistochemistry-Paraffin: Nogo-A Antibody [NBP2-41070] - Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 ug/mL.
Nogo-A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41070] -

Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41070] - Immunofluorescence of Nogo-A in Mouse Brain cells with Nogo-A antibody at 20 ug/mL.

Applications for Nogo-A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Despite its predicted molecular weight, Nogo-A typically migrates at 180 kDa in an SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nogo-A

Nogo-A, also known as Reticulon 4 (RTN4) is a transmembrane protein that is mainly expressed in oligodendrocytes of the central nervous system but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages, and myoblasts. Binding of the Nogo66 region to the Nogo Receptor/p75 complex on axons induces growth cone collapse and blocks neurite outgrowth. Nogo-A interactions with the Nogo-B receptor (NgBR/NUS1) can regulate vascular remodeling, inhibit neuronal differentiation, and promote glial formation from neural progenitors.

Long Name

Reticulon 4A

Alternate Names

NI220, NogoA, RTN4, RTN4A

Gene Symbol

RTN4

Additional Nogo-A Products

Product Documents for Nogo-A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nogo-A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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