NMT1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32168
![Knockdown Validated: NMT1 Antibody [NBP2-32168]](https://resources.bio-techne.com/images/products/NMT1-Antibody-Knockdown-Validated-NBP2-32168-img0004.jpg "Western Blot: NMT1 Antibody [NBP2-32168]")
![Western Blot: NMT1 Antibody [NBP2-32168]](https://resources.bio-techne.com/images/products/NMT1-Antibody-Western-Blot-NBP2-32168-img0001.jpg "Western Blot: NMT1 Antibody [NBP2-32168]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 68 to 118 of human N-Myristoyltransferase 1 using the numbering given in entry NP_066565.1 (GeneID 4836).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NMT1 Antibody - BSA Free
Western Blot: NMT1 Antibody [NBP2-32168]
Western Blot: NMT1 Antibody [NBP2-32168] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: NMT1 Antibody [NBP2-32168]](https://resources.bio-techne.com/images/products/NMT1-Antibody-Immunoprecipitation-NBP2-32168-img0003.jpg "Immunoprecipitation: NMT1 Antibody [NBP2-32168]")
Immunoprecipitation: NMT1 Antibody [NBP2-32168]
Immunoprecipitation: NMT1 Antibody [NBP2-32168] - Detection of human NMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for IP at 6 ug per reaction. NMT1 was also immunoprecipitated by rabbit anti-NMT1 antibody NBP2-32167. For blotting immunoprecipitated NMT1, NBP2-32168 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for NMT1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific publication (PMID: 32686708)
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NMT1
Alternate Names
alternative, short form NMT-S, glycylpeptide N-tetradecanoyltransferase 1, long form, NMT-L, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, NMTEC 2.3.1.97, N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase
Gene Symbol
NMT1
UniProt
Additional NMT1 Products
Product Documents for NMT1 Antibody - BSA Free
Product Specific Notices for NMT1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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