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NMDAR1 Antibody (R1JHL) - Azide and BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB300-118

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NB300-118

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # R1JHL

Format

Azide and BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for NMDAR1 Antibody (R1JHL) - Azide and BSA Free

Immunogen

Fusion protein containing amino acids 1-564 of the NMDAR1 subunit. Accession # P35439

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for NMDAR1 Antibody (R1JHL) - Azide and BSA Free

Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (https://www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.

Applications for NMDAR1 Antibody (R1JHL) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1 ug per ml

Immunoprecipitation

3ug/200ug lysate

Western Blot

1:1000
Application Notes
Use in ICC/IF and IHC-F reported in scientific literature (PMID: 20414717). Use in IHC-P reported in scientific literature (PMID 26635527).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 14 reviews rated 4.2 using NB300-118 in the following applications:

Published Applications

Read 20 publications using NB300-118 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Reconstitution

Reconstitute with 50 ul PBS to desired concentration.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR1

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Alternate Names

glutamate [NMDA] receptor subunit zeta-1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1glutamate [NMDA] receptor subunit zeta 1, NMD-R1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NR1

Entrez Gene IDs

24408 (Rat)

Gene Symbol

GRIN1

UniProt

Additional NMDAR1 Products

Product Documents for NMDAR1 Antibody (R1JHL) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NMDAR1 Antibody (R1JHL) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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