NLRP1/NALP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54899
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to NLRP1(NLR family, pyrin domain containing 1) The peptide sequence was selected from the N terminal of NLRP1 (NP_001028225). DTQEPRIVILQGAAGIGKSTLARQVKEAWGRGQLYGDRFQHVFYFSCREL. The peptide sequence for this immunogen was taken from within the described region.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 30342528)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for NLRP1/NALP1 Antibody - BSA Free
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899]
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] -
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] Human MCF7 whole cell. Antibody Dilution: 1ug/mlWestern Blot: NLRP1/NALP1 Antibody - BSA Free [NBP1-54899] -
RTP801 silencing in the R6/1 mouse hippocampal neurons reduces the levels of the inflammasome components. (a) Immunoblottings for NLRP1, cleaved NLRP1, procaspase 1, cleaved caspase 1, ASC/TMS1, and GFP as loading control for transduced neurons in the dorsal hippocampus of 4.5-month-old WT shCt, WT shRTP801, R6/1 shCt, and R6/1 shRTP801 mice. (b–f) Densitometric quantification of NRLP1 (treatment effect: F(1, 26) = 24.89, p < 0.0001; interaction: F(1, 26) = 1.175, p = 0.2883), cleaved NLRP1 (treatment effect: F(1, 29) = 44.18, p < 0.0001; genotype effect: F(1, 29) = 15.32, p = 0.0005; interaction: F(1, 29) = 6.588, P = 0.0157), ASC/TMS1 (treatment effect: F(1, 31) = 40.72, p < 0.0001; genotype effect: F(1, 31) = 17.85, p = 0.0002; interaction: F(1, 31) = 15.91, p = 0.0004), procaspase 1 (treatment effect: F(1, 30) = 26.06, p < 0.0001; interaction: F(1, 30) = 10.82, p = 0.0026) and cleaved caspase 1 (treatment effect: F(1, 30) = 25.57, p < 0.0001; interaction: F(1, 30) = 5.551, p = 0.0252) as in (a) for the hippocampus. Densitometric quantification of all proteins is expressed as the mean +/- SEM. All data were analyzed by two-way ANOVA followed by Bonferroni’s post hoc test. * p < 0.05, ** p < 0.01, and *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053183), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for NLRP1/NALP1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1.0 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-Frozen reported in scientific literature (PMID 24008734).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NLRP1/NALP1
Long Name
NLR Family, Pyrin Domain Containing 1
Alternate Names
CARD7, CLR17.1, DEFCAP, NALP1, PP1044, SLEV1, VAMAS1
Entrez Gene IDs
22861 (Human)
Gene Symbol
NLRP1
UniProt
Additional NLRP1/NALP1 Products
Product Documents for NLRP1/NALP1 Antibody - BSA Free
Product Specific Notices for NLRP1/NALP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...