NGFI-B alpha/Nur77/NR4A1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56745
![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]](https://resources.bio-techne.com/images/products/NGFI-B-alpha-Nur77-NR4A1-Antibody-Western-Blot-NB100-56745-img0005.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial synthetic peptide made to an internal portion of the human NGFI-B alpha protein (between amino acids 200-300) [UniProt P22736].
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NGFI-B alpha/Nur77/NR4A1 Antibody - BSA Free
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]
NGFI-B-alpha-Nur77-NR4A1-Antibody-Western-Blot-NB100-56745-img0005.jpg![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]](https://resources.bio-techne.com/images/products/NGFI-B-alpha-Nur77-NR4A1-Antibody-Immunohistochemistry-Paraffin-NB100-56745-img0007.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]")
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of NGFI-B alpha/Nur77/NR4A1 antibody (NB100-56745). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]](https://resources.bio-techne.com/images/products/NGFI-B-alpha-Nur77-NR4A1-Antibody-Western-Blot-NB100-56745-img0003.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]")
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745]
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
Applications for NGFI-B alpha/Nur77/NR4A1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
1:20-1:1000. Use reported in scientific literature (Lee et al (2009))
Chromatin Immunoprecipitation (ChIP)
1:20-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000. Use reported in scientific literature (Chintharlapalli et al (2005))
Immunohistochemistry
1:200. Use reported in scientific literature (PMID 31115481)
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:20-1:1000. Use reported in scientific literature (Ferlini et al (2009))
Western Blot
1:500-1:1000
Reviewed Applications
Read 3 reviews rated 4.7 using NB100-56745 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NGFI-B alpha/Nur77/NR4A1
Long Name
Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1
Alternate Names
HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3
Gene Symbol
NR4A1
UniProt
Additional NGFI-B alpha/Nur77/NR4A1 Products
Product Documents for NGFI-B alpha/Nur77/NR4A1 Antibody - BSA Free
Product Specific Notices for NGFI-B alpha/Nur77/NR4A1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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