NFIB Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81000
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24553933).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFIB Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]](https://resources.bio-techne.com/images/products/NFIB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81000-img0009.jpg "Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]")
Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]
Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.![Western Blot: NFIB Antibody [NBP1-81000]](https://resources.bio-techne.com/images/products/NFIB-Antibody-Western-Blot-NBP1-81000-img0007.jpg "Western Blot: NFIB Antibody [NBP1-81000]")
Western Blot: NFIB Antibody [NBP1-81000]
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.![NFIB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NFIB Antibody - BSA Free [NBP1-81000]](https://resources.bio-techne.com/images/products/nbp1-81000_rabbit-polyclonal-nfib-antibody-2562025911698.jpg "Chromatin Immunoprecipitation-exo-Seq: NFIB Antibody - BSA Free [NBP1-81000]")
Chromatin Immunoprecipitation-exo-Seq: NFIB Antibody - BSA Free [NBP1-81000]
ChIP-Exo-Seq composite graph for Anti-NFIB (NBP1-81000) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for NFIB Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFIB
Alternate Names
CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein
Entrez Gene IDs
4781 (Human)
Gene Symbol
NFIB
UniProt
Additional NFIB Products
Product Documents for NFIB Antibody - BSA Free
Product Specific Notices for NFIB Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...