NF2/Merlin Antibody (23GB2620)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51054
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2620
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human NF2/Merlin (Uniprot: P35240)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NF2/Merlin Antibody (23GB2620)
Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (23GB2620)[NBP3-51054] -
NF2/Merlin antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: NF2 / Merlin shRNA knockdown (KD). Nuclei were stained blue with DAPI; NF2 / Merlin was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (23GB2620)[NBP3-51054] -
Immunocytochemical staining of HAP-1 cells with NF2/Merlin antibody (1:1,000). Nuclei were stained blue with DAPI; NF2/Merlin was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: NF2/Merlin Antibody (23GB2620)[NBP3-51054] -
Validation of NF2/Merlin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NF2/Merlin / Merlin antibody (1:2,000) and analyzed using BD flow cytometer.
Applications for NF2/Merlin Antibody (23GB2620)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NF2/Merlin
Long Name
Neurofibromin 2
Alternate Names
ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin
Gene Symbol
NF2
Additional NF2/Merlin Products
Product Documents for NF2/Merlin Antibody (23GB2620)
Product Specific Notices for NF2/Merlin Antibody (23GB2620)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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