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Neuroligin 1/NLGN1 Antibody

Catalog # NBP2-76348 | Novus Biologicals a Bio-Techne Brand
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NBP2-76348

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine

Applications

Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for Neuroligin 1/NLGN1 Antibody

Immunogen

Peptide with sequence C-TAKQDDPKQQ corresponding to internal region according to NP_055747.1.

Marker

post-Synaptic Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Neuroligin 1/NLGN1 Antibody

Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Neuroligin 1/NLGN1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 150 kDa band in human brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa band according to NP_055747.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neuroligin 1/NLGN1

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

Alternate Names

Nlg, NLGN1

Gene Symbol

NLGN1

Product Documents for Neuroligin 1/NLGN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neuroligin 1/NLGN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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