Nestin Antibody (CL0197) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59775

Novus Biologicals, part of Bio-Techne
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NBP2-59775
NBP2-59775-25ul
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-44499AF350
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Alexa Fluor 405
NBP3-44499AF405
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Alexa Fluor 488
NBP3-44499AF488
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Alexa Fluor 532
NBP3-44499AF532
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Alexa Fluor 594
NBP3-44499AF594
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Alexa Fluor 647
NBP3-44499AF647
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Alexa Fluor 700
NBP3-44499AF700
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Alexa Fluor 750
NBP3-44499AF750
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Alexa Fluor Plus 488
NBP3-44499AFP488
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Alexa Fluor Plus 555
NBP3-44499AFP555
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Alexa Fluor Plus 594
NBP3-44499AFP594
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Alexa Fluor Plus 647
NBP3-44499AFP647
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Alexa Fluor Plus 680
NBP3-44499AFP680
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Allophycocyanin
NBP3-44499APC
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Biotin
NBP3-44499B
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CoraFluor 1
NBP3-44499CL1
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DyLight 350
NBP3-44499UV
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DyLight 405
NBP3-44499V
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DyLight 488
NBP3-44499G
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DyLight 550
NBP3-44499R
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DyLight 594
NBP3-44499DL594
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DyLight 650
NBP3-44499C
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DyLight 680
NBP3-44499FR
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DyLight 755
NBP3-44499IR
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FITC
NBP3-44499F
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HRP
NBP3-44499H
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Janelia Fluor 525
NBP3-44499JF525
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Janelia Fluor 549
NBP3-44499JF549
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Janelia Fluor 646
NBP3-44499JF646
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Janelia Fluor 669
NBP3-44499JF669
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mFluor Violet 450 SE
NBP3-44499MFV450
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mFluor Violet 500 SE
NBP3-44499MFV500
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mFluor Violet 610 SE
NBP3-44499MFV610
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PE
NBP3-44499PE
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PerCP
NBP3-44499PCP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0197

Format

BSA Free

Concentration

1 mg/ml

View all Nestin Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48681, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nestin Antibody (CL0197) - BSA Free

Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775] - Staining of RH-30 cells using the Anti-NES monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.
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Applications for Nestin Antibody (CL0197) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody (CL0197) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nestin Antibody (CL0197) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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