NEK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48625
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NEK1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit NEK1 Antibody (NBP3-48625) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NEK1 Antibody
NEK1 Antibody-NBP3-48625
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000.
NEK1 Antibody-NBP3-48625
U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nek1 protein stained by Nek1 antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 μm.
NEK1 Antibody-NBP3-48625
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for NEK1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NEK1
Alternate Names
DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2
Gene Symbol
NEK1
Additional NEK1 Products
Product Specific Notices for NEK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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