NEDD4 Antibody (23GB2255)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50248
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2255
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human NEDD4 (Uniprot: P46934)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEDD4 Antibody (23GB2255)
Immunocytochemistry/ Immunofluorescence: NEDD4 Antibody (23GB2255)[NBP3-50248] -
Immunocytochemical staining of HepG2 cells with NEDD4 antibody (1:1,000). Nuclei were stained blue with DAPI; NEDD4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: NEDD4 Antibody (23GB2255)[NBP3-50248] -
Flow cytometric analysis of NEDD4expression in HepG2 cells using NEDD4 antibody (1:2,000). Green, isotype control; red, NEDD4.
Western Blot: NEDD4 Antibody (23GB2255)[NBP3-50248] -
Western blotting analysis using NEDD4 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for NEDD4 Antibody (23GB2255)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NEDD4
Long Name
Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4
Alternate Names
RPF1
Gene Symbol
NEDD4
Additional NEDD4 Products
Product Documents for NEDD4 Antibody (23GB2255)
Product Specific Notices for NEDD4 Antibody (23GB2255)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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