NDUFB8 Antibody (6T8G1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15882
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6T8G1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFB8 (O95169). MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSPTPVSWHVMCMQLFGFLAFMIFMCWVGDVY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit NDUFB8 Antibody (6T8G1) (NBP3-15882) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NDUFB8 Antibody (6T8G1)
![Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]](https://resources.bio-techne.com/images/products/NDUFB8-Antibody-6T8G1-Western-Blot-NBP3-15882-img0005.jpg "Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]")
Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]
Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882] - Western blot analysis of extracts of various cell lines, using NDUFB8 Rabbit mAb (NBP3-15882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]](https://resources.bio-techne.com/images/products/NDUFB8-Antibody-6T8G1-Western-Blot-NBP3-15882-img0006.jpg "Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]")
Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882]
Western Blot: NDUFB8 Antibody (6T8G1) [NBP3-15882] - Western blot analysis of extracts of various cell lines, using NDUFB8 Rabbit mAb (NBP3-15882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: NDUFB8 Antibody (6T8G1) [NDUFB8] -
Immunohistochemistry: NDUFB8 Antibody (6T8G1) [NDUFB8] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NDUFB8 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for NDUFB8 Antibody (6T8G1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:1000 - 1:3000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NDUFB8
Alternate Names
CI-ASHImitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (19kD, ASHI), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa, NADH:ubiquinone oxidoreductase ASHI subunit, NADH-ubiquinone oxidoreductase ASHI subunit
Gene Symbol
NDUFB8
Additional NDUFB8 Products
Product Documents for NDUFB8 Antibody (6T8G1)
Product Specific Notices for NDUFB8 Antibody (6T8G1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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