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NDUFB11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83956

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NBP1-83956-25ul
NBP1-83956

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDYRMKEWSRREAERLVKYREANGLPIMESNCFDPSKIQLPEDE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFB11 Antibody - BSA Free

Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP1-83956]

Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP1-83956]

Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP1-83956] - Staining of human duodenum shows strong, granular cytoplasmic positivity in glandular cells.
NDUFB11 Antibody - BSA Free Western Blot: NDUFB11 Antibody - BSA Free [NBP1-83956]

Western Blot: NDUFB11 Antibody - BSA Free [NBP1-83956]

Analysis in U2OS cells) transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NDUFB11 antibody. Remaining relative intensity is presented.
NDUFB11 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: NDUFB11 Antibody [NBP1-83956]

Immunocytochemistry/ Immunofluorescence: NDUFB11 Antibody [NBP1-83956]

Staining of human cell line U-2 OS shows localization to mitochondria.

Applications for NDUFB11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFB11

NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 (PubMed 12381726)).(supplied by OMIM)

Alternate Names

CI-ESSS, complex I NP17.3 subunit, Complex I-ESSS, ESSS, FLJ20494, MGC111182, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial, NADH-ubiquinone oxidoreductase ESSS subunit, Neuronal protein 17.3, Np15, NP17.3, P17.3

Gene Symbol

NDUFB11

Additional NDUFB11 Products

Product Documents for NDUFB11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDUFB11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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