NDUFAF1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84503
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQSALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAF
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NDUFAF1 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody [NBP1-84503]](https://resources.bio-techne.com/images/products/NDUFAF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84503-img0006.jpg "Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody [NBP1-84503]")
Immunocytochemistry/ Immunofluorescence: NDUFAF1 Antibody [NBP1-84503]
Immunocytochemistry/Immunofluorescence: NDUFAF1 Antibody [NBP1-84503] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.![NDUFAF1 Antibody - BSA Free Western Blot: NDUFAF1 Antibody - BSA Free [NBP1-84503]](https://resources.bio-techne.com/images/products/nbp1-84503_rabbit-polyclonal-ndufaf1-antibody-104202515393791.jpg "Western Blot: NDUFAF1 Antibody - BSA Free [NBP1-84503]")
Western Blot: NDUFAF1 Antibody - BSA Free [NBP1-84503]
Analysis in control (vector only transfected HEK293T lysate) and NDUFAF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414253).Applications for NDUFAF1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NDUFAF1
Alternate Names
CGI-65, CIA30CGI65, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
Gene Symbol
NDUFAF1
Additional NDUFAF1 Products
Product Documents for NDUFAF1 Antibody - BSA Free
Product Specific Notices for NDUFAF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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