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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for NCOA4 Antibody - BSA Free

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.

Predicted Species

Canine (100%), Equine (100%), Monkey (100%). Backed by our 100% Guarantee.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA4 Antibody - BSA Free

Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Immunocytochemistry/Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with NBP3-18136 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for NCOA4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

1 ug/ml

Western Blot

0.5-1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCOA4

The NCOA4 gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gen

Alternate Names

Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator

Gene Symbol

NCOA4

Product Documents for NCOA4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCOA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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