Nav1.7 Antibody (N68/6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12904
![Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Immunocytochemistry-Immunofluorescence-NBP2-12904-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]")
![Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Western-Blot-NBP2-12904-img0013.jpg "Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Hamster
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # N68/6
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7
Localization
Membrane , Synapse
Specificity
Detects approx 230kDa. No cross-reactivity against other Nav channels.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Nav1.7 Antibody (N68/6) - BSA Free
Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904]
Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904] - Western Blot analysis of Hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Immunocytochemistry-Immunofluorescence-NBP2-12904-img0017.jpg "Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]")
Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]
Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.7 Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.7 Monoclonal Antibody (NBP2-12904) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.7 Antibody (D) Composite.![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0016.jpg "Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]")
Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]
Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0014.jpg "Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]")
![Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]](https://resources.bio-techne.com/images/products/Nav1-7-Antibody-N68-6-Immunohistochemistry-NBP2-12904-img0015.jpg "Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]")
Applications for Nav1.7 Antibody (N68/6) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of Nav1.7 Antibody was sufficient for detection of Nav1.7 in 10 ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nav1.7
Long Name
Sodium channel protein type 9 subunit alpha
Alternate Names
hNE-Na, Ne-Na, NENA, PN1, SCN9A
Gene Symbol
SCN9A
Additional Nav1.7 Products
Product Documents for Nav1.7 Antibody (N68/6) - BSA Free
Product Specific Notices for Nav1.7 Antibody (N68/6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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