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NAP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26592

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NBP1-26592
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human TAK1 binding protein 3 (mitogen-activated protein kinase kinase kinase 7 interacting protein 3) using the numbering given in entry NP_690000.2 (GeneID 257397)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAP1 Antibody - BSA Free

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. TAB3 was also immunoprecipitated by rabbit anti-TAB3 antibody NBP1-26593.
Immunoprecipitation: NAP1 Antibody [NBP1-26592]

Immunoprecipitation: NAP1 Antibody [NBP1-26592]

Immunoprecipitation: NAP1 Antibody [NBP1-26592] - Detection of human TAB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 (lot NBP1-26592-2) used for IP at 6 ug per reaction. TAB3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26592-1). For blotting immunoprecipitated TAB3, NBP1-26592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.

Applications for NAP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NAP1

TAK1-binding protein 3 (TAB3) is a binding and regulatory subunit of the transforming growth factor-beta-activated kinase 1 (TAK1). TAB3 functions as an adaptor that mediates IL1 and TNF signaling pathways. Additionally it plays a role in the activation of the NF-kappaB pathway by functioning as receptors that bind polyubiquitin chains for the regulation of kinase activity.

Alternate Names

CG5330, Dmel\CG5330, dNap, dNAP1, dNAP-1, MAP3K7IP3, MGC45404, mitogen-activated protein kinase kinase kinase 7 interacting protein 3, Mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NAP1, Nap-1, NF-kappa-B-activating protein 1, NFkB activating protein 1, p56/dCAF-4, TAB-3, TAK1-binding protein 3, TGF-beta activated kinase 1/MAP3K7 binding protein 3, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, TGF-beta-activated kinase 1-binding protein 3

Entrez Gene IDs

257397 (Human)

Gene Symbol

TAB3

Additional NAP1 Products

Product Documents for NAP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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