Nanog Antibody - BSA Free

Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842]

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Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842]

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Immunohistochemistry: Nanog Antibody [NB100-58842]

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Immunoprecipitation: Nanog Antibody [NB100-58842]

Immunoprecipitation: Nanog Antibody [NB100-58842] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody. For blotting immunoprecipitated Nanog, this was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842]

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot: Nanog Antibody [NB100-58842] -

Western Blot: Nanog Antibody [NB100-58842] - The forced expression of Tlk1 results in the aberrant downregulation of core pluripotency factors & attenuates self-renewal. (A) Immunoblot analysis of Oct4, Sox2, & Nanog levels in control mESCs (empty vector or doxycycline depletion) & Tlk1-overexpressing mESCs. The mESCs expressing an empty vector or the Tet-On-Tlk1 or Tet-On-Tlk1-D607A expression vector were cultured in the absence or presence of doxycycline (Dox; 100 ng/ml) for 24 hrs under undifferentiated self-renewal conditions. (B) Quantification of results from (A). The protein levels of the target genes were normalized to alpha-tubulin levels. The protein expression levels of each mESC line not treated with doxycycline were normalized to 1. The biological data are presented as mean (n = 6) ± SEM. *Р < 0.05, & **P < 0.01. (C) The morphology & AP staining of Tet-On-inducible Tlk1-expressing cell lines cultured in mock (Dox−) or doxycycline (Dox+) for 48 hrs. Scale bar, 500 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] -

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha-tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] -

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha-tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] -

Western Blot: Nanog Antibody [NB100-58842] - Tlk1 is not required for mESC self-renewal or pluripotency. (A) The efficiency of Tlk1 knockdown (KD) in control (shLuc) & Tlk1-KD mESCs (shTlk1 #1 & #2) was confirmed by RT-qPCR analysis. Data are mean (n = 3) ± SEM. **Р < 0.01 & ***Р < 0.001. (B) The morphology of control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was evaluated using phase-contrast microscopic images & AP staining. Scale bars represent 500 µm. (C & D) The mRNA expression of pluripotency-associated & development-associated genes were analyzed by RT-qPCR in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs cultured under undifferentiated self-renewal conditions. All data were normalized to Gapdh & plotted relative to the expression level in control cells. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. (E) The protein levels of pluripotency factors in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was analyzed by immunoblotting using antibodies specific to Oct4, Sox2, & Nanog. (F) Quantification based on densitometry of Western blotting data from (E). All data were normalized to alpha-tubulin. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] -

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha-tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] -

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha-tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.