Nanog Antibody (CL5810) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61429
![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]](https://resources.bio-techne.com/images/products/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0002.jpg "Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]")
![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]](https://resources.bio-techne.com/images/products/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0003.jpg "Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]")
![Western Blot: Nanog Antibody (CL5810) [NBP2-61429]](https://resources.bio-techne.com/images/products/Nanog-Antibody-CL5810-Western-Blot-NBP2-61429-img0006.jpg "Western Blot: Nanog Antibody (CL5810) [NBP2-61429]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL5810
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from Q9H9S0, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Nanog Antibody (CL5810) - BSA Free
Western Blot: Nanog Antibody (CL5810) [NBP2-61429]
Western Blot: Nanog Antibody (CL5810) [NBP2-61429] - Analysis in human cell line NTERA-2.![Immunocytochemistry/ Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429]](https://resources.bio-techne.com/images/products/Nanog-Antibody-CL5810-Immunocytochemistry-Immunofluorescence-NBP2-61429-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429]")
Immunocytochemistry/ Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429]
Immunocytochemistry/Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429] - Staining of NTERA-2 cells using the Anti-NANOG monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]](https://resources.bio-techne.com/images/products/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0004.jpg "Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]")
Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]
Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429] - Staining of human testis shows absence of positivity in seminiferous ducts as expected (negative control).Applications for Nanog Antibody (CL5810) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbol
NANOG
Additional Nanog Products
Product Documents for Nanog Antibody (CL5810) - BSA Free
Product Specific Notices for Nanog Antibody (CL5810) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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