N6-methyladenosine (m6A) Antibody (C.15200082) - BSA Free

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171]

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Immunoprecipitation was performed on two radiolabelled synthetic DNA oligos, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the monoclonal m6A antibody. The immunoprecipitated fraction is shown in lane 3. Lane 2 shows the non bound fraction, whereas the input is shown in lane 1.

Dot Blot: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligos were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10% skimmed milk and 1% BSA. Figure shows a high specificity of the antibody for the modified oligonucleotide.

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171]

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing 6mA nucleotides, using 2 ug of the monoclonal m6A antibody. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure shows the recovery, expressed as % input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).