Skip to main content

Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

Catalog # NBP2-47900 | Novus Biologicals a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-47900-0.1mg

Key Product Details

Species Reactivity

Human, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MYH11/923

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Summary for Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

Immunogen

Recombinant full-length human Myosin heavy chain 11 protein (Uniprot: P35749)

Reactivity Notes

Predicted to have broad species reactivity.

Localization

Cytoplasmic

Specificity

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Marker

Leiomyosarcoma & Myoepithelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44533).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free [NBP2-47900] - Human Leiomyosarcoma stained with SM-MHC Monoclonal Antibody (MYH11/923).
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free [NBP2-47900] - Human Angiosarcoma stained with SM-MHC Monoclonal Antibody (MYH11/923).
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free [NBP2-47900] - Human Colon Carcinoma stained with SM-MHC Monoclonal Antibody (MYH11/923).

Applications for Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

: 0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

: 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Myosin heavy chain 11

The protein encoded by the MYH11 gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. MYH11 functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

AAT4, FAA4, MYH11, MYH11 myosin, heavy chain 11, smooth muscle, SMHC, SMMHC, Smooth muscle myosin heavy chain

Gene Symbol

MYH11

Product Documents for Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myosin heavy chain 11 Antibody (MYH11/923) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...