MyoD Antibody (HL1372) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25573
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (95%), Porcine (95%), Sheep (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1372 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human MyoD. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MyoD Antibody (HL1372) - Azide and BSA Free (NBP3-25573) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MyoD Antibody (HL1372) - Azide and BSA Free
Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] -
Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] -
Immunocytochemistry/Immunofluorescence: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Immunohistochemistry-Paraffin: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] -
Immunohistochemistry-Paraffin: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for MyoD Antibody (HL1372) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MyoD
Long Name
Myoblast Determination Protein 1
Alternate Names
bHLHc1, MYF3, MYOD1, PUM
Gene Symbol
MYOD1
Additional MyoD Products
Product Documents for MyoD Antibody (HL1372) - Azide and BSA Free
Product Specific Notices for MyoD Antibody (HL1372) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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