ZNRF3 (Zinc/RING Finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).

References

de Lau, W. et al. (2014) Genes Dev. 28:305.
Xie, Y. et al. (2013) EMBO Rep. 14:1120.
Peng, W.C. et al. (2013) PLoS One 8:e83110.
Hao, H.X. et al. (2012) Nature 485:195.
Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
Koo, B.K. et al. (2012) Nature 488:665.
Ong, C.K. et al. (2012) Nat. Genet. 44:690.
Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
Ryland, G.L. et al. (2013) J. Pathol. 229:469.
Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.

Long Name

Zinc and Ring Finger 3

Alternate Names

BK747E2.3, KIAA1133, RNF203

Entrez Gene IDs

84133 (Human); 407821 (Mouse)

Gene Symbol

ZNRF3

UniProt

Q5SSZ7

Additional ZNRF3 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse ZNRF3 Alexa Fluor™ Plus 488-conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Citations
Reviews

Mouse ZNRF3 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8328AFP488

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ZNRF3 Antibodies »

Product Specifications

Specificity

Clonality

Host

Isotype

Applications for Mouse ZNRF3 Alexa Fluor™ Plus 488-conjugated Antibody

Immunocytochemistry

Background: ZNRF3