Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Lymphotactin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse XCL1/Lymphotactin Biotinylated Antibody

XCL1/Lymphotactin in Mouse Thymus.

XCL1/Lymphotactin was detected in perfusion fixed frozen sections of mouse thymus using Mouse XCL1/Lymphotactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF486) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Mouse XCL1 / Lymphotactin ELISA Standard Curve

Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) was serially diluted and captured by Rat Anti-Mouse XCL1/Lymphotactin Monoclonal Antibody (Catalog # MAB486) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse XCL1/Lymphotactin Biotinylated Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)

Mouse XCL1/Lymphotactin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Mouse XCL1/Lymphotactin Antibody (Catalog # MAB486)
Standard: Recombinant Mouse XCL1/Lymphotactin Protein (Catalog # 486-LT)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

References

Kelner, G. et al. (1994) Science 266:1395.
Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Long Name

Chemokine [C Motif] Ligand 1

Alternate Names

ATAC, Lymphotactin, SCM-1 alpha

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Gene Symbol

XCL1

Additional XCL1/Lymphotactin Products

Product Documents for Mouse XCL1/Lymphotactin Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse XCL1/Lymphotactin Biotinylated Antibody

For research use only

Citations
Reviews

Mouse XCL1/Lymphotactin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF486

Key Product Details

Species Reactivity

Validated:

Cited:

Applications