Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑3a and rmWnt‑5a is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Wnt-1 Antibody

Wnt-1 in Mouse Embryonic Spinal Cord.

Wnt-1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Wnt-1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Wnt-1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-1

Wnt-1 is a secreted glycoprotein that binds members of the frizzled family of seven transmembrane domain receptors. Wnt-1 may have an important role in the morphogenesis of the neural tube and in CNS development.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Gene Symbol

WNT1

UniProt

Q3UR96

Additional Wnt-1 Products

Product Documents for Mouse Wnt-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Wnt-1 Antibody

For research use only

Citations
Reviews

Mouse Wnt-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1620

Key Product Details

Species Reactivity

Validated:

Cited:

Applications