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Mouse Wnt‑1 Antibody

Catalog # AF1620 | R&D Systems, Inc. a Bio-Techne Brand
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AF1620
AF1620-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse Wnt‑1 Antibody

Immunogen

E. coli-derived recombinant mouse Wnt‑1 peptide
Ser52-Glu92 and Met234-Arg313
Accession # Q3UR96

Specificity

Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑3a and rmWnt‑5a is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Wnt‑1 Antibody

Wnt‑1 antibody in Mouse Embryonic Spinal Cord by Immunohistochemistry (IHC-Fr).

Wnt‑1 in Mouse Embryonic Spinal Cord.

Wnt-1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Wnt‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Wnt‑1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF1620 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-1

Wnt-1 is a secreted glycoprotein that binds members of the frizzled family of seven transmembrane domain receptors. Wnt-1 may have an important role in the morphogenesis of the neural tube and in CNS development.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Gene Symbol

WNT1

UniProt

Product Documents for Mouse Wnt‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Wnt‑1 Antibody

For research use only

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Reconstitution Calculator

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