VG5Q was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse VG5Q Monoclonal Antibody (Catalog # MAB30481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse VG5Q Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VG5Q

Angiogenic Factor VG5Q, also known as AGGF1, is a presumably secreted protein expressed by vascular endothelial cells. Mouse VG5Q cDNA encodes a 714 aa precursor with a coiled coil domain, a forkhead (FHA) domain, and RNA-association domains (G-patch, OCRE). VG5Q binds the angiogenic TNF superfamily ligand TWEAK, and promotes endothelial cell proliferation. Human VG5Q mutations are associated with Klippel-Trenaunay syndrome, a congenital vascular morphogenesis disorder. Mouse VG5Q shares 78% aa identity with human VG5Q.

Long Name

Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1

Alternate Names

AGGF1

Entrez Gene IDs

55109 (Human)

Gene Symbol

AGGF1

UniProt

Q7TN31

Additional VG5Q Products

Product Documents for Mouse VG5Q Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse VG5Q Antibody

For research use only

Citations
Reviews

Mouse VG5Q Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB30481

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse VG5Q Antibody

VG5Q in bEnd.3 Mouse Cell Line.