Mouse TWEAK R/TNFRSF12 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB16101R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 314636
Product Specifications
Immunogen
E. coli-derived recombinant mouse TWEAK R
Glu28-Trp79
Accession # Q9CR75.1
Glu28-Trp79
Accession # Q9CR75.1
Specificity
Detects mouse TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) EDA, rmNeogenin, recombinant human TWEAK R, rmOPG, or rmTNF R1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse TWEAK R/TNFRSF12 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: MS-1 mouse pancreatic islet endothelial cell line
Sample: MS-1 mouse pancreatic islet endothelial cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TWEAK R/TNFRSF12
TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2‑6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).
References
- Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
- Feng, S. et al. (2000) Am. J. Pathol. 156:1253.
- Wiley, S. et al. (2001) Immunity 15:837.
- Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
- Nakayama, M. et al. (2002) J. Immunol. 168:734.
- Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Alternate Names
CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR
Gene Symbol
TNFRSF12A
UniProt
Additional TWEAK R/TNFRSF12 Products
Product Documents for Mouse TWEAK R/TNFRSF12 Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Mouse TWEAK R/TNFRSF12 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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