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Mouse TSLP Antibody

Catalog # MAB555 | R&D Systems, Inc. a Bio-Techne Brand
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MAB555-100
MAB555-500
MAB555-SP

Key Product Details

Species Reactivity

Mouse

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 152614

Product Summary for Mouse TSLP Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6

Specificity

Detects mouse TSLP in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TSLP Antibody

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.

Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND50 is typically 0.1-0.4 µg/mL.
Detection of Mouse TSLP by Block/Neutralize

Detection of Mouse TSLP by Block/Neutralize

TSLP neutralization inhibits airway structural alterations.(A) The depth of peribronchial collagen deposition was significantly reduced in anti-TSLP-treated mice, compared with HDM-exposed and IgG isotype-treated controls. (B) Epithelial goblet cells were markedly reduced in anti-TSLP-treated mice, compared to the HDM-exposed and IgG -treated controls. (C) TGF-beta 1 levels in the BALF were significantly increased in HDM-exposed mice. The blockage of TSLP with the anti-TSLP mAb reduced TGF-beta 1levels in the BALF. TGF-beta 1 protein and mRNA levels were determined in duplicate experiments. (D-G) Representative photomicrographs of Masson's trichrome-stained lung sections from mice that were exposed for 5 weeks to saline (D) or HDM (E) and pretreated with anti-TSLP mAb (F) or an IgG-treated control (G) 60 minutes prior to and during HDM exposure. (H-K) Representative photomicrographs of airway sections of PAS-stained from mice that were exposed for 5 weeks to saline (H) or HDM (I) and pretreated with anti-TSLP mAb (J) or an IgG-treated control (K) 60 minutes prior to and during the HDM exposure. Original magnification, 20×. The data shown represent the means±SEM (n = 5), **p<0.01 compared with the HDM group, ##p<0.01 compared with the IgG-treated control mice. The photomicrographs of H-K are representative of 5 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051268), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TSLP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using MAB555 in the following applications:

Published Applications

Read 25 publications using MAB555 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Product Documents for Mouse TSLP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TSLP Antibody

For research use only

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