Detects mouse Tryptase beta‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Tryptase alpha and rhTryptase beta‑2 is observed and no cross-reactivity with recombinant mouse MCPT-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Tryptase beta-1/MCP-7/Mcpt7 (Catalog # 1937-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase beta-1/MCP-7

Tryptase beta-1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (MCP-7 or Mcpt7) (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid (aa) sequence for mouse Tryptase beta-1 consists of a signal peptide (aa 1‑18), a pro region (aa 19‑28) and the mature chain (aa 29‑273).

References

McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Long Name

Mast Cell Protease 7

Alternate Names

Mast Cell Tryptase, MCP-7, Mcp7, Mcpt7, TPSB1, TRYB1, Tryptase beta1

Entrez Gene IDs

100503895 (Mouse)

Gene Symbol

TPSAB1

UniProt

Q02844

Additional Tryptase beta-1/MCP-7 Products

Product Documents for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

For research use only

Citations
Reviews

Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1937

Key Product Details

Species Reactivity

Validated:

Cited:

Applications