Detects mouse Tryptase‑5/Prss32 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tryptase-G1, recombinant mouse (rm) MCPT-6, and rmMCPT‑7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse Tryptase-5/Prss32 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Tryptase-5/Prss32 (Catalog # 2634-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Tryptase-5/Prss32 (Catalog # 2634-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Tryptase-5/Prss32 (2 µg/mL, Catalog # 2634-SE) cleavage of the fluorogenic peptide substrate Z-R-SBzl (100 µM). The Neutralization Dose (ND₅₀) is typically 24.3 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase-5/Prss32

Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome.

References

Wong, G. W. et al. (2004) J. Biol. Chem. 279:2438.

Alternate Names

Prss32, Tryptase5

Entrez Gene IDs

69814 (Mouse)

Gene Symbol

PRSS32

UniProt

NP_081496

Additional Tryptase-5/Prss32 Products

Product Documents for Mouse Tryptase-5/Prss32 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Tryptase-5/Prss32 Antibody

For research use only

Citations
Reviews

Mouse Tryptase-5/Prss32 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2634

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Tryptase-5/Prss32 Antibodies »

Product Specifications

Immunogen

Specificity