Mouse Testican 3/SPOCK3 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2346S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
Ala26-Ile436
Accession # Q8BKV0
Ala26-Ile436
Accession # Q8BKV0
Specificity
Detects mouse Testican 3/SPOCK3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human (rh) Testican 3 is observed, less than 5% cross‑reactivity with rhTestican 1 i
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Testican 3/SPOCK3
Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). The human and mouse cDNAs predict 90% identity between the deduced amino acid sequences from the two species, indicating a conserved function (2, 3). Only the human protein, but not the mouse protein, has been characterized in the literature. Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to testicans, is responsible for the inhibition of Testican 3 towards MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca2+-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1). The purified rmTestican 3 is capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). As compared to rhTestican 1 (R&D Systems, Catalog # 2327-PI), the IC50 of rmTestican 3 is weaker toward rhCathepsin L and rhMMP-14 activity.
Alternate Names
SPOCK3
Gene Symbol
SPOCK3
UniProt
Additional Testican 3/SPOCK3 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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