Mouse Syndecan-3 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2734AFP488

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FAB2734AFP488-100UG
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 312607

View all Syndecan-3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3

Specificity

Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) Syndecan-1, rmSyndecan-4, or recombinant human Syndecan-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Syndecan-3

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of mouse Syndecan-3 shares 95%, 83%, 82% and 81% aa identity with of rat, human, canine and bovine Syndecan-3, respectively. Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

References

  1. Xian, X. et al. (2010) Cell Tissue Res. 339:31.
  2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
  3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
  4. ENTREZ protein Accession # Q64519.
  5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
  7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
  8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
  9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
  10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
  11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
  12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
  13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
  14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Alternate Names

N-Syndecan, SDC3, Syndecan3

Entrez Gene IDs

9672 (Human); 20970 (Mouse)

Gene Symbol

SDC3

UniProt

Q64519

Additional Syndecan-3 Products

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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