Mouse Spi-B Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7204AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Spi-B
Specificity
Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse Spi-B Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Spi-B
Spi-B (Transcription factor Spi-B) is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. Mouse Spi-B is 267 amino acids (aa) in length. It contains a dual transactivation region (aa 1-62), plus a PEST domain (aa 110-170) and an Ets DNA-binding domain (aa 174-257). There are two isoform variants. One shows a nine aa substitution for aa 1-8, while a second possesses an 18 aa insertion after Leu17. Over aa 18-167, mouse Spi-B shares 91% and 74% aa identity with rat and human Spi-B, respectively.
Long Name
Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B
Alternate Names
SpiB
Gene Symbol
SPIB
UniProt
Additional Spi-B Products
Product Specific Notices for Mouse Spi-B Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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