Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serpin A3N Antibody

Serpin A3N in Mouse Brain Tissue.

Serpin A3N + labelled oligodendrocytes can be seen in the Corpus callosum of cuprizone-intoxicated mice. Primary antibody: Serpin A3N 1:1000, 18h incubation at 4°CSecondary antibody: rabbit anti goat, 1:200, 21°C, 1h incubation. Visualization via DAB. Image from a verified customer review.

Detection of Mouse Serpin A3N by Western Blot

HS diet increased the serpina3n expression in the hippocampus of APP/PS1 mice. (A) GSEA showed that the HS diet intervention changed the status of axon guidance, neuroactive ligand–receptor interaction, oxidoreductase activity, and blood vessel diameter. (B) A radar map of differential gene expression levels. Serpina3n is most significantly changed, and its mRNA expression is the highest among the serpin family. (C) Protein level of serpina3n was assessed using Western blotting; GAPDH was used as a control. n = 4 biologically independent animals. (D) mRNA levels of the serpin family in HS- and ND-treated APP/PS1 mice were compared; serpina3n is the most remarkably changed mRNA. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11731), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Serpin A3N Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin A3N (Catalog # 4709-PI)

Reviewed Applications

Read 2 reviews rated 5 using AF4709 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Immunohistochemistry-Paraffin (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

References

Forsyth, S. et al. (2003) Genomics 81:336.
Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.

Alternate Names

Spi2

Entrez Gene IDs

20716 (Mouse); 24795 (Rat)

Gene Symbol

SERPINA3N

UniProt

Q91WP6 (Mouse)

Additional Serpin A3N Products

Product Documents for Mouse Serpin A3N Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse Serpin A3N Antibody

For research use only

Citations
Reviews

Mouse Serpin A3N Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4709

Key Product Details

Species Reactivity

Validated:

Cited:

Applications