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Mouse S100A10 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # AF2377
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Key Product Details
| Species Reactivity: | Mouse |
| Immunogen: |
E. coli-derived recombinant mouse S100A10 Pro2-Lys97 Accession # P08207 |
| Applications: | Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Polyclonal Goat IgG |
| Specificity: |
Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed. |
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Western BlotDetection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunohistochemistryS100A10 in Mouse Kidney. S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
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Simple WesternDetection of Mouse S100A10 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Mouse S100A10 Antibody (12)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse S100A10 Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Hwang et al. (2022), Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice Journal of Neuroinflammation, 19(1):142.
PMID: 35690821 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotB Hou et al. (2020), Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype Cell Death Dis, 11(5):377.
PMID: 32415059 -
Species: Mouse
Sample Types: WholeTissue
Applications: IHCD Sargin et al. (2020), Mapping the physiological and molecular markers of stress and SSRI antidepressant treatment in S100a10 corticostriatal neurons Mol. Psychiatry, 25(5):1112-1129.
PMID: 31431686 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotC Zuo et al. (2020), Neuroprotective efficacy of different levels of high-frequency repetitive transcranial magnetic stimulation in mice with CUMS-induced depression: Involvement of the p11/BDNF/Homer1a signaling pathway J Psychiatr Res, 125(0):152-163.
PMID: 32289652 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJ Jin et al. (2019), Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior Mol. Psychiatry, 0(0).
PMID: 30760886 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotG Liu et al. (2019), P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice Int. J. Biol. Sci., 15(7):1383-1395.
PMID: 31337969 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCJ Yamada et al. (2019), PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy J. Neurosci., 0(0).
PMID: 31801810 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FT Vanmierlo et al. (2018), Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels Mol. Cell. Neurosci., 0(0).
PMID: 29501719 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Sajgo et al. (2017), Molecular codes for cell type specification in Brn3 retinal ganglion cells Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28465430 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCSchmidt EF et al. (2012), Identification of the cortical neurons that mediate antidepressant responses. Cell, 149(5):1152-63.
PMID: 22632977 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotHanaue M et al. (2011), Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract. Mol. Reprod. Dev., 78(2):91-103.
PMID: 21254279 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotWarner-Schmidt JL et al. (2009), Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation. J. Neurosci., 29(6):1937-46.
PMID: 19211900
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