Mouse/Rat DLL1 Antibody

Name: Mouse/Rat DLL1 Antibody
Brand: R&D Systems
SKU: AF5026
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF5026

Citations (5)

(1)

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Species Reactivity:	Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse DLL1 Ser22-Gln516 Accession # Q61483
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects mouse and rat DLL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) DLL1 and less than 1% cross-reactivity with rhDLL3, rhDLL4, and recombinant mouse DLL4 is observed.

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Applications for Mouse/Rat DLL1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse DLL1 Fc Chimera (Catalog # 5026-DL) Recombinant Rat DLL1 Fc Chimera (Catalog # 3970-DL)
Immunohistochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse/Rat DLL1 Antibody

	Immunohistochemistry DLL1 in Embryonic Mouse Kidney. DLL1 was detected in immersion fixed frozen sections of embryonic mouse kidney (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5026) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90‑100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6‑12). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (14‑15).

Item	Value
References	Bettenhausen, B. et al. (1995) Development 121:2407. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Shimojo, H. et al. (2008) Neuron 58:52. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Citations for Mouse/Rat DLL1 Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat DLL1 Antibody include:

Species: Chicken
Sample Types: Cell Lysates
Applications: Western Blot

T Nomura et al. (2022), Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Nature Communications, 13(1):96.
PMID: 35013223
Species: Mouse
Sample Types: Embryo
Applications: IHC

S Ofek et al. (2021), Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Bmc Biology, 19(1):84.
PMID: 33892704
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F

S Wiszniak et al. (2019), Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 20(1):21.
PMID: 31036074
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

E Vasilaki et al. (2018), Dll1 Marks Cells of Origin of Ras-Induced Cancer in Mouse Squamous Epithelia Transl Oncol, 11(5):1213-1219.
PMID: 30081298
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Toru Nakata et al. (2017), Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine. Data in Brief, 0(0):2352-3409.
PMID: 28124021

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Customer Reviews for Mouse/Rat DLL1 Antibody (1)

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Mouse/Rat DLL1 Antibody

Name: Anonymous

Application: It worked very good for western blot.

Sample Tested: Brain (cortex) tissue,brain cortex

Species: Mouse

Verified Customer | Posted 08/06/2021

1:500 dilution is good.

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Mouse/Rat DLL1 Antibody

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Applications for Mouse/Rat DLL1 Antibody

Scientific Data Examples for Mouse/Rat DLL1 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: DLL1

Citations for Mouse/Rat DLL1 Antibody (5)

Customer Reviews for Mouse/Rat DLL1 Antibody (1)

Customer Images

Mouse/Rat DLL1 Antibody

Product Documents for Mouse/Rat DLL1 Antibody

Product Datasheets

COA

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