Mouse Rae-1 alpha / beta /δ/ epsilon Antibody

Name: Mouse Rae-1 alpha / beta /δ/ epsilon Antibody
Brand: R&D Systems
SKU: MAB17581
Price: 111 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB17581

Citations (3)

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Species Reactivity:	Mouse
Immunogen:	BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58)
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 199205
Specificity:	Detects mouse Rae‑1 alpha / beta /δ/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.

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Applications for Mouse Rae-1 alpha / beta /δ/ epsilon Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-6 ng/mL of this antibody will block 50% of the binding of 300 ng/mL of Biotinylated Recombinant Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1δ Fc Chimera (Catalog # 1134‑RA) coated at 1 µg/mL (100 µL/well). At 100 ng/mL, this antibody will block >90% of the binding. Blocking of Recombinant Mouse Rae-1 beta is observed at a similar antibody concentration, however, this antibody does not block the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha or Rae-1 epsilon in this assay.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Item	Value
Long Name	Retinoic Acid Early Transcript 1
Entrez Gene IDs	8480 (Human); 19368 (Mouse)
Alternate Names	Rae-1, Rae1, dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1

Citations for Mouse Rae-1 alpha / beta /δ/ epsilon Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Rae-1 alpha / beta /δ/ epsilon Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012-01-01), Ras activation induces expression of Raet1 family NK receptor ligands. J. Immunol., 189(4):1826-34.
PMID: 22798674
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Yoshida S et al. (2012-01-01), Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair. J. Immunol., 188(8):3972-9.
PMID: 22403443
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Klezovich-Benard M et al. (2012-01-01), Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog., 8(1):e1002481.
PMID: 22253596

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Mouse Rae-1 alpha / beta /δ/ epsilon Antibody

R&D Systems, Inc. a Bio-Techne Brand

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