Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Protogenin in Mouse Neural Tube.

Protogenin was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Protogenin in Mouse Embryo.

Protogenin was detected in immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Protogenin Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E9) and immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Protogenin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protogenin

Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.

Alternate Names

IGDCC5, PRTG, RPTG, Shen-Dan

Entrez Gene IDs

283659 (Human); 235472 (Mouse); 315806 (Rat)

Gene Symbol

PRTG

UniProt

Q2EY15

Additional Protogenin Products

Product Documents for Mouse Protogenin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Protogenin Antibody

For research use only

Citations
Reviews

Mouse Protogenin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4919

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Protogenin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Protogenin Antibody

Protogenin in Embryonic Mouse Neural Tube.