Mouse pIgR Antibody

Name: Mouse pIgR Antibody
Brand: R&D Systems
SKU: AF2800

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2800

Citations (15)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse pIgR Lys19-Lys645 Accession # O70570
Applications:	Block/Neutralize, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.

View Full Technical Details for Mouse pIgR Antibody

Applications for Mouse pIgR Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of mouse IgM to immobilized Recombinant Mouse pIgR (Catalog # 2800-PG) coated at 5 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse pIgR Antibody

	Western Blot Detection of Mouse pIgR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry pIgR in Mouse Liver. pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: pIgR

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

Item	Value
References	de Groot, N. et al. (1999) Transgenic Res. 8:125. Piskurich, J. et al. (1995) J. Immunol. 154:1735. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35. Asano, M. et al. (2004) Immunology 112:583. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55. Uren, T. et al. (2003) J. Immunol. 170:2531.
Long Name	Polymeric Immunoglobulin Receptor
Entrez Gene IDs	5284 (Human); 18703 (Mouse)
Alternate Names	FLJ22667, Hepatocellular carcinoma-associated protein TB6, MGC125361, MGC125362, Poly-Ig receptor, hepatocellular carcinoma associated protein TB6, pIgR, polymeric immunoglobulin receptor

Citations for Mouse pIgR Antibody (15)

Showing 1 - 10 of 15 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse pIgR Antibody include:

Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

B Zeng et al. (2020), Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein Nutrients, 12(9).
PMID: 32911830
Species: Human
Sample Types: Whole Tissue
Applications: IHC

AM Collin et al. (2020), Lung immunoglobulin A immunity dysregulation in cystic fibrosis EBioMedicine, 60(0):102974.
PMID: 32927272
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

M Basic et al. (2018), Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation Sci Rep, 8(1):719.
PMID: 29335601
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

C Bornstein et al. (2018), Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells Nature, 559(7715):622-626.
PMID: 30022162
Species: Mouse
Sample Types: BALF

KM Azzam et al. (2017), Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces JCI Insight, 2(16).
PMID: 28814662
Species: Plant
Sample Types: Crude Leaf Extracts
Applications: ELISA Development (Capture)

K Nakanishi et al. (2017), Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA Sci Rep, 7(0):45843.
PMID: 28368034
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Moon C et al. (2015), Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation. Nature, 521(7550):90-3.
PMID: 25686606
Olsan E et al. (2015), Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease. J Biol Chem, 290(25):15679-86.
PMID: 25922073
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Hegab et al. (2014), Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation. Stem Cells Dev, 23(6):664-75.
PMID: 24171691
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Moon C et al. (2014), Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol, 7(4):818-28.
PMID: 24220295
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Louten J et al. (2012), Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 7(0):87-104.
PMID: 22837640
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Frantz A et al. (2012), Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol, 5(5):501-12.
PMID: 22491177
Species: Mouse
Sample Types: Mucosal Lavage Fluid
Applications: ELISA Development

Libanova R et al. (2010), The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant. Vaccine, 28(10):2249-58.
PMID: 20060510
Species: Mouse
Sample Types: BALF
Applications: ELISA Development

Shim DH et al. (2010), Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens. Vaccine, 28(11):2311-7.
PMID: 20060944
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Kang X et al. (2010), Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid. Proteomics, 10(3):520-31.
PMID: 20029843

There are no citations that match your criteria.

Showing 1 - 10 of 15 citations Showing All

Product Documents for Mouse pIgR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse pIgR Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Mouse pIgR Antibody

Scientific Data Examples for Mouse pIgR Antibody

Western Blot

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: pIgR

Citations for Mouse pIgR Antibody (15)

Product Documents for Mouse pIgR Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected