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Mouse Patched 2/PTCH2 Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8096AFP647

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FAB8096AFP647-100UG

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 865619

Product Specifications

Immunogen

E. coli-derived recombinant mouse Patched 2/PTCH2

Specificity

Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Patched 2/PTCH2

Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. It is less widely expressed than Patched 1, but is found at high levels in skin and spermatocytes. The 1182 amino acid (aa) mouse Patched 2 contains two terminal cytoplasmic domains between aa 1-57 and aa 1108-1182. Together with Smoothened (SMO), Patched 2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (Shh). Over the first extracellular loop (aa 79-394), mouse Patched 2 shares 95% aa sequence identity with human Patched 2. Mouse Patched 2 has one potential 1117 aa isoform that lacks aa 207-271.

Alternate Names

ptc2, PTCH2

Entrez Gene IDs

8643 (Human); 19207 (Mouse)

Gene Symbol

PTCH2

UniProt

Additional Patched 2/PTCH2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

Recommended applications are based on validated applications from the unconjugated base product MAB8096.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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