NKX3.1 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse NKX3.1 Monoclonal Antibody (Catalog # MAB6475) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse NKX3.1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX3.1

NKX3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. NKX3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse NKX3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mouse NKX3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Entrez Gene IDs

4824 (Human); 18095 (Mouse); 305999 (Rat)

Gene Symbol

NKX3-1

UniProt

P97436

Additional NKX3.1 Products

Product Documents for Mouse NKX3.1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse NKX3.1 Antibody

For research use only

Citations
Reviews

Mouse NKX3.1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6475

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NKX3.1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse NKX3.1 Antibody

NKX3.1 in NIH‑3T3 Mouse Cell Line.