Mouse NGFR/TNFRSF16 Antibody

Detection of Mouse NGF R/TNFRSF16 by Western Blot.

Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NGF R/TNFRSF16 in Mouse Brain.

NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse NGFR/TNFRSF16 by Immunohistochemistry

Immunohistochemistry validation of bilateral stellate ganglion neuronal subtypes identified from scRNAseq data analysis. Except NA2 and Glut, all other neuronal subtypes express either high or low Npy. NA1a, NA1b, NA1c neurons appeared numerically more compared to the NA2, NA3, ACh1, ACh2, and Glut. (A) NA1a neuronal subtypes in the stellate ganglia. NA1a neurons express high Cntn5 and Npy (Inset) and can be easily distinguished from the low Npy expressing neurons co-labeled with Cntn5high. (B) Stellate section contains neurons that stained for Kcnt1 and express either low or high Npy but the NA1b subtype can be distinguished by low Npy and high Kcnt1 expression (inset). (C) NA1c subtype similar to NA1b contains low Npy but higher Sctr labeled on the cell borders (inset). (D) NA2 neurons are differentiated from the Glut with expression of high Calm1 and low Ngfr while Glut neuronal subtype expresses low Calm1 and high Ngfr. (E) NA3 subtype can be separated from ACh1 based on low Npy and high Vimentin gene expression whereas ACh1 (F); express high Npy and low Vimentin. (G) ACh2 subtype was separated from others with low Npy and high Vip expression. (H) Gluts expressed high Ngfr expression and are negative for Cntn5 and Sst. In insets, all subtypes are marked for each group and their magnified view is shown in the upper right corner of the figure. n=6. Scale bars 50 and 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37162194), licensed under a CC-BY license. Not internally tested by R&D Systems.