Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody

Name: Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: FAB6689R

R&D Systems, part of Bio-Techne | Catalog # FAB6689R

Submit a review

Product Datasheet / COA

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP Leu1587-Ser2229 Accession # Q8VHY0
Applications:	Immunohistochemistry
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Specificity:	Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.

View Full Technical Details for Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: NG2/MCSP

NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes. It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha. Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein. The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size. Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety). There are two splice variants. One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667. Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.

Item	Value
Long Name	Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs	1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names	AN2, CSPG4, EC 3.6.3, HMW-MAA, MCSP, MCSPChondroitin sulfate proteoglycan NG2, MCSPG, MCSPGMelanoma chondroitin sulfate proteoglycan, MEL-CSPG, MSK16Melanoma-associated chondroitin sulfate proteoglycan, NG2, NG2EC 2.7.8, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)

Customer Reviews

There are currently no reviews for this product. Be the first to review Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody and earn rewards!

Have you used Mouse NG2/MCSP Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Product Documents

Product Datasheets

Download Product Datasheets