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Mouse Netrin‑G1a Antibody

Catalog # AF1166 | R&D Systems, Inc. a Bio-Techne Brand
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AF1166
AF1166-SP

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse Netrin‑G1a Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Netrin-G1a
His29-Gly513
Accession # Q8R4G0.2

Specificity

Detects mouse Netrin-G1a in direct ELISAs and Western blots. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Netrin‑G1a Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Netrin-G1a (Catalog # 1166-NG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using AF1166 in the following applications:

Published Applications

Read 5 publications using AF1166 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G1a

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-G1a is synthesized as a 539 amino acid (aa) precursor with an 18 aa signal sequence, a 399 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 122 aa C domain with a hydrophobic signal for glycosyl phosphatidylinositol (GPI) lipid linkage. Unlike classical Netrins which have a C domain rich in basic aa residues that serves as a heparin binding site, Netrin-G1a is predominantly anchored on the cell surface via GPI linkages. By alternative splicing, at least six isoforms for Netrin-G1 have been identified. All G1a variants (G1b through G1f) are shorter than G1a and lack one or more of the EGF-like domains. Mouse Netrin-G1a shares 27%, 26%, and 31% aa sequence identity with mouse Netrins-1, 3 and 4, respectively. It also shares 60% aa identity with another GPI-anchored mouse Netrin-G2a. Although a human homolog of mouse Netrin-G1a has not been reported, a human G1f that shares 97% aa sequence identity with mouse G1f has been cloned. Netrin-G1 has widespread expression, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion). The G-type Netrins are found only in vertebrates and are suggested to play a role in forming circuits associated with motor activity. Conditioned media containing myc-tagged Netrin-G1a proteins did not bind to cells transiently transfected with the Netrin receptors DCC, UNC5H1, UNC5H2, or UNC5H3 (1‑4).

References

  1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
  2. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
  3. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.
  4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.

Alternate Names

NetrinG1a, NTG1, NTNG1

Entrez Gene IDs

22854 (Human); 80883 (Mouse)

Gene Symbol

NTNG1

UniProt

Product Documents for Mouse Netrin‑G1a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Netrin‑G1a Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

For research use only

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