Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha₈ beta₁(1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down‑regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha₈ beta₁(2). Since deletion of integrin alpha₈ beta₁ results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha₈ beta₁ ligand during Nephrogenesis (2, 3).

References

Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
Miner, J. H. (2001) J. Cell Biol. 154:257.

Alternate Names

EGFL6L, Nctn, NPNT, POEM

Entrez Gene IDs

255743 (Human); 114249 (Mouse)

Gene Symbol

NPNT

UniProt

AAH68308

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Product Specific Notices

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

Recommended applications are based on validated applications from the unconjugated base product AF4298.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Mouse Nephronectin Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4298AFP488

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Background: Nephronectin