Detects mouse MMP-12 in direct ELISAs and Western blots. In direct ELISAs,less than 20% cross-reactivity with recombinant human MMP‑12 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-8, and rmMMP-10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MMP-12 Antibody

Detection of Mouse MMP-12 by Western Blot.

Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-12 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse MMP-12 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse MMP‑12 (Catalog # 3467‑MP), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Mouse serum and mouse plasma

Reviewed Applications

Read 1 review rated 5 using AF3467 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-12

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-12 (macrophage elastase) is found in macrophages and its expression in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling. In addition to elastin, MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha₁-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. MMP-12 is required for macrophage-mediated proteolysis and matrix invasion in mice. MMP-12 is proposed to have a direct role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. Structurally, the pro-MMP-12 consists of following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The recombinant mouse MMP-12 corresponds to the pro form that can be activated.

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Entrez Gene IDs

4321 (Human); 17381 (Mouse)

Gene Symbol

MMP12

UniProt

EDL24933

Additional MMP-12 Products

Product Documents for Mouse MMP-12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse MMP-12 Antibody

For research use only

Citations
Reviews

Mouse MMP-12 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3467

Key Product Details

Species Reactivity

Validated:

Cited:

Applications