Detects Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TPS-1 and rhTPS-B2 is observed and less then 10% cross-reactivity with recombinant mouse MCP-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease-6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Mast Cell Protease-6/Mcpt6 (Catalog # 3736-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (0.5 µg/mL, Catalog # 3736‑SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002 ). The Neutralization Dose (ND₅₀) is typically 3 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

References

Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535 .
Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.

Alternate Names

Mast Cell Protease6, Mcpt6

Entrez Gene IDs

7177 (Human); 17229 (Mouse); 29268 (Rat)

Gene Symbol

TPSAB1

UniProt

P21845

Additional Mast Cell Protease-6/Mcpt6 Products

Product Documents for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Mast Cell Protease-6/Mcpt6 Antibody

For research use only

Citations
Reviews

Mouse Mast Cell Protease-6/Mcpt6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3736

Key Product Details

Species Reactivity

Validated:

Cited:

Applications